FastQCFastQC Report
Wed 6 Jul 2022
HN7TMAFX3_n02_as4-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7TMAFX3_n02_as4-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519239
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC76990.2187688872509085Illumina Single End PCR Primer 1 (100% over 50bp)
CGCTAAATAAGGACATGACACATGCGAATCATACGTTTATTATTGATAAG56090.15938104800498062No Hit
TTTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG44260.1257658260777401Illumina Single End PCR Primer 1 (100% over 45bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG43530.12369151398924597Illumina Single End PCR Primer 1 (100% over 48bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG42670.12124780385759536Illumina Single End PCR Primer 1 (100% over 48bp)
TTTGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG41020.11655929023291682Illumina Single End PCR Primer 1 (100% over 45bp)
TGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG37820.10746641532444941Illumina Single End PCR Primer 1 (100% over 45bp)
TGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG37650.10698335634493708Illumina Single End PCR Primer 1 (100% over 45bp)
TTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG37320.10604565362000136Illumina Single End PCR Primer 1 (100% over 45bp)
CAATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG37190.10567625557684487Illumina Single End PCR Primer 1 (100% over 45bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT36370.10334620638155011Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35880.10195385991119102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)