FastQCFastQC Report
Wed 6 Jul 2022
HN7TMAFX3_n01_as4-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7TMAFX3_n01_as4-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6230045
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT71290.11442935002877186Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC68460.10988684672422108Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTA63510.10194147875336375TruSeq Adapter, Index 13 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)