FastQCFastQC Report
Wed 6 Jul 2022
HN7TMAFX3_n01_as4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7TMAFX3_n01_as4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7470772
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT161130.2156805213704822Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC159870.2139939486842859Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC106920.14311773937151342TruSeq Adapter, Index 3 (100% over 44bp)
ACACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC104730.14018631541693416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC99020.1325431963390129TruSeq Adapter, Index 3 (100% over 44bp)
CACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA95710.12811259666337027Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC92100.12328043206244281TruSeq Adapter, Index 3 (100% over 44bp)
CATAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC91930.12305287860478141TruSeq Adapter, Index 3 (100% over 44bp)
TATTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC88520.11848842395404385TruSeq Adapter, Index 3 (100% over 44bp)
ACGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC86190.11536960303433166TruSeq Adapter, Index 3 (100% over 44bp)
TTACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC83170.11132718278646436TruSeq Adapter, Index 3 (100% over 44bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTA83090.11122009880638842TruSeq Adapter, Index 3 (100% over 47bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTA82050.10982800706540101TruSeq Adapter, Index 3 (100% over 47bp)
GCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC78000.10440688057405581TruSeq Adapter, Index 3 (100% over 44bp)
ACCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT77170.10329588428076776TruSeq Adapter, Index 3 (100% over 43bp)
TTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC77010.10308171632061587TruSeq Adapter, Index 3 (100% over 44bp)
TTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC76660.10261322390778356TruSeq Adapter, Index 3 (100% over 44bp)
TTCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT76080.10183686505223288TruSeq Adapter, Index 3 (100% over 43bp)
TACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC75980.10170301007713795TruSeq Adapter, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)