FastQCFastQC Report
Wed 6 Jul 2022
HN7TMAFX3_n01_as4-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7TMAFX3_n01_as4-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519239
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG72560.20618093854949893TruSeq Adapter, Index 1 (100% over 49bp)
CAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC42640.12116255815532848TruSeq Adapter, Index 1 (100% over 44bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA42280.12013960972812587TruSeq Adapter, Index 1 (100% over 47bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA42120.11968496598270251TruSeq Adapter, Index 1 (100% over 47bp)
TCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC39510.11226858988548376TruSeq Adapter, Index 1 (100% over 44bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT38720.11002378639245586Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC36330.10323254544519427TruSeq Adapter, Index 1 (100% over 44bp)
CATAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC36270.1030620540406605TruSeq Adapter, Index 1 (100% over 44bp)
ACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC35940.10212435131572478TruSeq Adapter, Index 1 (100% over 44bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC35860.1018970294430131Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC35610.10118664859078909TruSeq Adapter, Index 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)