FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_y3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_y3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22125928
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306650.1385930569782203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG26250.015.070930558
CGCGGGT27600.014.3184961
GTTACGG27550.014.23321659
TACGGTC29000.013.64126561
TTACGGT30000.013.30370260
CTCGAAT29850.012.0675991
GACCTAG45850.011.74902255
CGGGTCT60900.011.7161033
ACCTAGC39600.011.6602786
GTTCGAT16700.011.5179241
ACGCTTA31000.011.40518844
CTCGGGT46400.011.3811721
GCGATAG27350.011.26346556
CATTCGG24500.011.13612654
GCATTCG22300.011.1358593
CTCGGTT28150.010.9328231
CGCTTAA29750.010.82539245
CTCGGAT36350.010.7756041
CGACCGT10100.010.7341671
CTCGCAT27550.010.6631551
Produced by FastQC (version 0.11.2)