FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_y2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_y2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19874365
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261090.13137023497354505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT34950.013.808961
ACCCGAT33900.011.87184568
CCCGATA34400.011.4959469
CGGGTCT60350.011.4167973
TCGCTCG35650.011.38927914
CCGATAT33800.011.28576570
TCGGGGT55650.011.2485962
GTCCGAT14150.011.1220581
ACTTCGC41150.010.63044711
TGTTACG21600.010.53514858
CGCTTAA25750.010.33226345
CCGACCG39450.010.20176566
CTCGTAT12100.010.1160691
GCGGGTC51550.010.0402262
GCATTCG19550.010.0183593
ACTCGTG42600.09.94149522
AACCCGA43450.09.82647667
GTTACGG22100.09.82235259
TACTCGT42450.09.81231421
CTCGTAC42250.09.44524417
Produced by FastQC (version 0.11.2)