FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_y1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_y1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18539288
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444540.23978267126547687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT33500.013.6766471
CCGATAT32950.012.32035270
ACCCGAT33850.012.30297268
CCCGATA33950.011.95755269
TCGCTCG35750.011.84713114
CGTCGAT8900.011.396231
TCGGGGT52500.011.2580152
GCATTCG17750.011.2318973
ACTTCGC40750.010.90619411
CTCGGAT30750.010.8051711
GTCCGAT12750.010.4238121
CGGGTCT61350.010.3760823
ACTCGTG42300.010.25997822
TACTCGT40950.010.25678521
CTCGTAC41450.010.21866217
CATTCGG19350.09.7615224
CGCAGAC63850.09.6953951
AAACCCG44950.09.576766
AACCCGA44950.09.5766567
TCGGATT16700.09.4238962
Produced by FastQC (version 0.11.2)