FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_h3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_h3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24084337
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT39800.020.2109641
CGGGTCT74300.013.9810463
TCGCTCG39600.012.63999714
GCGGGTC70750.012.0116392
ACCCGAT42100.011.97025668
ACTTCGC44200.011.71738911
ACGGAAC67950.011.69276534
GTTACGG25750.011.42085159
CCCGATA42650.011.2412569
CCGATAT44500.011.00970670
CTCGGGT42750.010.9625341
GACGGAA72900.010.9469633
CTCGGAT42900.010.8426791
TGTTACG25950.010.65756958
CCGACCG69150.010.62849566
GTTCGAT19850.010.3952221
TCGTGCG48000.010.20621424
TACTCGT48000.010.06308921
CTCGTAC49650.010.01182317
TCGTACT54750.09.8464118
Produced by FastQC (version 0.11.2)