FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_h2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_h2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31275843
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT45550.020.2695451
CGGGTCT84800.014.0648223
TCGCTCG46150.013.04522614
GCGGGTC77800.012.3615772
GTTACGG32400.011.77811559
CTCGGAT55150.011.5412891
ACTTCGC54700.011.38771111
CTCGGTT44150.011.0898521
GCGATAG35600.011.01313256
CCGACCG76150.010.75449666
TGTTACG34650.010.70970458
CCCGATA55350.010.68547269
ACCCGAT55850.010.65267668
CGATAGA38450.010.65216757
ACGGAAC80800.010.61302434
CGCAGAC80700.010.2707771
CTCGTAT20250.010.189561
TCGTGCG57850.010.16214424
CTTGGAT170950.010.14707851
CCGATAT59900.010.10730970
Produced by FastQC (version 0.11.2)