FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29257210
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424340.14503775308718775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT35650.016.7753941
CTCGGTT38550.013.0639421
CTCGTAT17700.013.0408761
TCGCTCG36550.013.0241114
CGGGTCT71900.012.9398433
CTCGGGT49800.012.7813921
GCGATAG34150.012.30064856
GTTACGG32750.012.29349359
CTCGTTT41650.012.2595351
CTCAGAT193150.012.2401891
CTCGGAT51850.012.141151
TAAGTCG24550.011.69193620
TTTGCGC45200.011.46116421
CTGCGTT73450.011.4276131
CGATAGA36950.011.36868457
TCGGGTC40950.011.1879122
TCGGATT30600.011.0861822
GGCGATA38300.010.96773555
CTCGAAC57250.010.8126931
TAGGCGA41000.010.67218253
Produced by FastQC (version 0.11.2)