FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25233188
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG799280.31675743865578937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG24650.012.0711556
GCATTCG22450.011.8396963
TAAGTCG19750.011.16595720
CATTCGG23850.010.8523254
CGGGGTT75750.010.5717961
CGATAGA28700.010.48978657
GGCGATA30000.010.26846455
GTTCGAT18450.09.4769721
TTACACG25450.09.352132
TCGGGGT62250.09.3253032
CTCGAAT29550.09.2306661
CTCGTAT13750.09.1557881
GTCCGAT16050.09.1510331
TACGTCG10350.09.12891624
CGATAAG27900.09.0336617
TAGGCGA35000.08.90127553
CTCGTTT31900.08.8795371
CTCGGTT29150.08.8774691
GACGCAA29700.08.72223944
ATAGGCG34150.08.71250452
Produced by FastQC (version 0.11.2)