FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22618310
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311780.13784407411517483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG68350.017.97762338
GTCCGAT15600.012.77953151
GCATTCG22450.011.3735043
GTTCGAT19200.011.2941911
AAGGGGG217500.010.7804569
CTCGTAT14400.010.6869761
CATTCGG24250.010.67425254
GCGATAG20350.010.32143156
TCGGGGT55850.010.2070962
CGATTGG16550.010.1452044
TAAGTCG17500.09.80094320
AAAAGGG248850.09.7462267
AAAGGGG241000.09.74385268
AGGGGGG258000.09.65770570
CGATAGA22900.09.47794357
CCGATTG20100.09.2229013
TTACACG24300.09.07428432
ATAGGGG58650.09.07273637
GGCGATA23950.09.06221755
GGGTTAA60700.09.05431154
Produced by FastQC (version 0.11.2)