FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19524319
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388240.19884944514582045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG25600.012.58005956
TAAGTCG18400.012.17500520
GGCGATA28550.011.52532655
CGATAGA28300.011.3799357
CTCGTAT13550.011.3577081
CATTCGG19650.011.2150434
GCATTCG20450.011.1177343
TTACACG18950.010.71283932
GTTCGAT16400.010.6635911
CTCGGGT40950.010.5911991
TCGGGTC28950.010.5103422
CTCGTTT28050.010.474271
CGATAAG24800.010.44483317
ATAGGCG31750.010.36329452
GACGCAA21300.010.35407644
CTCGGAT31150.010.3301611
CTCAGAT136950.010.1903291
CTTAGAT56850.09.8438931
CTTGGAT112450.09.82891
CGCGTAT2850.00344323169.8179871
Produced by FastQC (version 0.11.2)