FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30380010
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500470.1647366146357424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377750.12434163122395285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT44650.017.9373841
CGGGTCT82650.012.5686523
TCGCTCG47850.011.26510614
CGAATCG30200.011.01121852
TAAGTCG26450.010.98423120
GCGGGTC80150.010.9523152
ACGGAAC71000.010.64798834
ACTTCGC53000.010.36597211
ACCCGAT50050.010.34874268
CTCGGAT55800.010.21641351
CCCGATA51400.010.14475669
CTCGGGT48350.010.1269021
GTTCGAT27200.010.0293041
ACGATTG34850.09.84024626
GACGGAA76950.09.73357333
CCGACCG75750.09.65634766
CCGATAT54050.09.64700670
CGATAAG34650.09.5971917
GTCCGAT19000.09.3877531
CTCGGTT42250.09.3539871
Produced by FastQC (version 0.11.2)