FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29979930
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG736940.24581111430213481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT39500.019.7449461
TCGCTCG45200.012.93234414
CGGGTCT82350.012.0623293
ACCCGAT48200.011.98034768
CCCGATA49050.011.7725269
ACTTCGC52200.011.59754411
CCGATAT50300.011.47980970
TAAGTCG24650.011.36025920
CTCGGAT51150.011.0085071
ACGGAAC66850.010.41912634
TCGTGCG55400.010.35893324
TTATGCG29450.010.34168238
CTCGTAC56950.010.26494917
TACTCGT57300.010.26236321
CTCGGGT50900.010.1006141
CGATAAG30050.010.01816117
GCGGGTC76850.010.0116242
GCGATAG31400.09.92210556
GACGGAA71350.09.85992733
CTCAGAT180550.09.8404371
Produced by FastQC (version 0.11.2)