FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n02_asp1_c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n02_asp1_c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23779201
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG438670.1844763413202992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA348940.1467416840456498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT41700.019.039181
CGGGTCT73500.012.8491713
ACGGAAC60150.011.63758334
GCGGGTC70950.011.4361872
GACGGAA65400.010.70350933
CGCGTAT4351.2514029E-610.4523161
GTCCGAT16050.010.2419181
CCGACCG66300.09.87118166
CGCCCTT36550.09.5690991
TCGCTCG37400.09.54588514
TCCGACC73250.09.46137765
CTCGGTT30300.09.2343391
TCGGTAT14050.09.2102422
CCCGATA37700.09.18986769
AGATCCG75100.09.18315141
TTGGACG75500.09.17854530
ACCCGAT38150.09.17341268
TCGGATT25950.09.1646782
GTCGAAT12500.08.9536151
CTCGGAT43650.08.8940011
Produced by FastQC (version 0.11.2)