FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_y3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_y3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22125928
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT708070.3200182157331435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG27550.014.22807456
CGCGGGT29450.012.4897671
GGCGATA30600.012.35244855
CTCGGAT34500.012.1846341
TCGCTCG38800.012.08741914
CGATAGA32000.012.0304757
TTACACG20800.011.94663832
ACCCGAT37000.011.82358568
CCCGATA38450.011.5598569
GCATTCG23050.011.5420423
CCGATAT38600.011.51492870
CGCGAAA4652.1156666E-811.29137643
GTTCGAT18050.011.2564421
CGCTTAA28650.011.23991445
CGGGTCT60750.011.2364293
CATTCGG25600.011.2121884
ATAGGCG34650.011.21192652
CTCGGGT44700.011.1283591
TAGGCGA34400.011.08987353
ACTTCGC42750.010.88837911
Produced by FastQC (version 0.11.2)