FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_y2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_y2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19874365
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT726260.36542551170817283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG22800.039.14886543
CTCGTAT24550.036.07304842
TATGCCG25050.035.49204646
CGTATGC26050.034.5333944
TCTCGTA34200.026.09917641
ATGCCGT39450.022.62544847
AGTCACG40300.022.31919328
ACACGTC39800.022.24856813
ACGGCTA42400.022.03920232
ATCTCGT47100.019.91687240
ATCGGAA47500.018.6442092
GATCGGA48900.018.19521
ACGTCTG50800.018.1201215
CACGTCT52750.017.3838814
CGGCTAC52300.017.19815433
GCCGTCT53150.017.1875949
GTCACGG54550.016.93785929
TCACGGC58200.015.9357830
CGCGGGT33800.015.5455651
GTATGCC61100.015.46780745
Produced by FastQC (version 0.11.2)