FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_y1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_y1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18539288
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439470.23704793841057975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT32750.014.0117251
ACCCGAT34350.013.7545468
CTCGTAT10850.013.55007742
TCGTATG10850.013.55007743
CCGATAT34950.013.51837570
CCCGATA35250.013.40336169
TCGCTCG37450.012.71015314
ACTTCGC38100.012.40111511
TACTCGT40700.011.86646621
GCATTCG20850.011.7518753
CGGGTCT61900.011.4228883
CGTATGC13550.011.3666444
TATGCCG13600.011.32454546
CTCGGAT33000.011.2518411
TCGGGGT53150.011.1959782
ACTCGTG42600.011.17293422
CTCGTAC44250.010.99397317
CTCGGGT40950.010.6927081
TTACACG15300.010.52240132
AACCCGA45100.010.47590667
Produced by FastQC (version 0.11.2)