FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_h3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_h3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24084337
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT935120.3882689400999496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG22100.026.60938843
CGTATGC22850.025.42935244
CTCGTAT24650.023.43068142
CGCGGGT41050.021.5892411
TATGCCG28400.020.45915846
GTCACGA33700.017.44728329
TCTCGTA35900.016.47814641
ACGATCA38950.015.63485332
AGTCACG39300.014.78311128
CGGGTCT78900.014.7738963
ACACGTC40900.014.54702213
ATGCCGT42500.014.33028247
TCACGAT44950.013.62575130
GCGGGTC73700.012.966562
ACGGAAC67250.012.95822734
CGATCAG48750.012.70719833
GACGGAA71900.012.41259133
CCGACCG67600.011.95932166
CACGATC50700.011.87335631
CTCGGGT44550.011.6370811
Produced by FastQC (version 0.11.2)