FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_h2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_h2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31275843
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT874790.2797014935776472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT43350.018.747551
TCGCTCG48000.013.27042114
GTTACGG33750.012.96197959
CGGGTCT85750.012.8586343
TGTTACG34950.012.41683758
GCGGGTC77000.011.7286142
CTCGGTT46800.011.6019621
ACCCGAT54350.011.2688268
CCCGATA54800.011.24020269
CTCGGGT56200.011.1573821
CTCGGAT55750.011.0589381
CTCGCAT41550.010.9601871
ACTTCGC59750.010.9533711
TCGGTTA22850.010.8765172
ACGGAAC77850.010.83406434
GTTCGAT26000.010.6438651
CCGATAT58200.010.64365870
GCGATAG35850.010.54340356
GACCTAG68050.010.49303155
CTCGTTT42800.010.39454651
Produced by FastQC (version 0.11.2)