FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29257210
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1348260.4608299971186589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT33700.017.9830991
TCGTATG20500.017.0747543
CGTATGC23500.015.3418244
TCGCTCG38250.014.45702614
CTCGTAT25250.014.00127142
TTTGCGC42200.013.85002821
CTGCGTT72600.013.0761841
CTCGGAT48850.012.9079221
CGGGTCT73450.012.8675483
TATGCCG27250.012.84480946
CTCGGTT40050.012.7702291
GCGATAG35850.012.49602556
GTTCGAT22550.012.2723841
GCGTTCT59950.012.2617923
CTCGGGT49850.012.2273521
CGATAGA37150.011.964416557
CCCGATA45250.011.833528569
ACCCGAT44250.011.78460868
CCCGACT55100.011.698081
ACTTCGC46200.011.66625111
Produced by FastQC (version 0.11.2)