FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25233188
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920300.36471808476994666No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC443880.17591118490457883TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59800.053.73209846
TCGTATG60350.053.12777743
CTCGTAT61050.052.4612842
CGTATGC61700.051.79494544
TATCTCG65450.048.93330839
TCTCGTA72100.044.51880641
ACACGTC76600.042.4016813
AGTCACG80000.040.5104228
ATGCCGT80150.040.35127647
ATCTCGT80300.040.10289840
GATCGGA81300.039.7674751
CGCCAAT83450.038.66803433
GTCACGC87400.036.960229
ATCGGAA88900.036.5798872
CACGTCT93600.034.850314
ACGCCAA92600.034.847132
GCCGTCT93800.034.29132549
ACGTCTG95750.034.1043815
CCGTCTT101900.031.5999350
GCACACG104300.031.47583211
Produced by FastQC (version 0.11.2)