FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22618310
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1002940.44341951277526925TruSeq Adapter, Index 5 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501100.22154617210569666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG121750.062.8759246
TCGTATG121450.062.8013643
CTCGTAT120700.061.3355742
CGTATGC125100.061.19314244
TCTCGTA127450.058.2248341
ATGCCGT138300.055.70580347
GATCGGA139300.055.1722221
ACACGTC141300.054.6655613
ATCTCGT136550.054.39526740
ATCGGAA147100.052.544292
ACGTCTG152850.050.64906315
CACGTCT154400.050.0729314
GCCGTCT154200.049.686949
GCACACG161900.048.1416811
CCGTCTT160700.047.8514150
TCGGAAG164000.047.2361763
CACACGT164150.047.2260612
CGTCTGA164300.047.03393616
TGCCGTC165350.046.59173248
GATCTCG163400.045.5421839
Produced by FastQC (version 0.11.2)