FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19524319
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297190.15221529621596533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23200.038.7743346
CGTATGC23350.038.52593244
TCGTATG24200.036.8838543
CTCGTAT26050.033.1895842
TCTCGTA31700.027.715741
ATGCCGT36750.025.52531247
ACACGTC37000.024.97220213
AATCTCG36100.024.53065739
GATCGGA37800.024.0947461
ATCTCGT37950.023.70436540
ATCGGAA45000.020.3810652
ACGTCTG48500.018.97889315
CACGTCT49100.018.81820514
GCCGTCT49900.018.65745749
GCACACG54350.017.45116411
CCGTCTT56400.016.69338850
CGTCTGA56250.016.5505616
CACACGT56500.016.22963112
TCGGAAG57350.016.0531143
TGCCGTC58950.015.85298448
Produced by FastQC (version 0.11.2)