FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30380010
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1162620.38269243492678245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT46950.018.3539711
TCGTATG18500.012.8662443
CGGGTCT89700.012.7606233
TCGCTCG52050.012.036283514
ACTTCGC56100.011.91580711
ACCCGAT52850.011.65495668
CGTATGC22700.011.56499844
CCCGATA53950.011.41743369
CTCGTAT21500.011.39656642
ACGGAAC74400.010.96026134
CCGATAT57400.010.79209470
GCGGGTC81650.010.7605762
GTTCGAT23750.010.4718951
TAAGTCG27500.010.30846120
CTCGGGT56450.010.3008881
GACGGAA80400.010.186028533
AACCCGA64450.010.04595167
TACTCGT62400.010.03953421
CTCGTAC62450.09.97573317
CTCGGAT54350.09.7965811
Produced by FastQC (version 0.11.2)