FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29979930
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT672540.22433007682139353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT38050.017.4929521
TAAGTCG26250.012.66583420
TCGTATG15600.012.34082843
CGGGTCT81450.012.1198373
GTTCGAT24650.012.079981
CTCGGTT40700.011.8781521
CTCGGAT51600.011.4057481
CTCGGGT54600.011.3565081
GCGATAG30900.011.21311856
TCGCTCG42850.011.18989414
CGATAAG30200.011.1254517
GCGGGTC70950.010.8051792
CGCGAAA7301.8189894E-1210.54887243
CGATAGA33700.010.48894357
GGCGATA35950.010.41682455
CTGGAAT225600.010.3729321
CGTATGC19100.010.26266544
CTCGTTT42850.010.0558431
TTATGCG29050.010.00061138
TCGGTTA18750.09.8949792
Produced by FastQC (version 0.11.2)