FastQCFastQC Report
Mon 23 Nov 2015
HN7T2BGXX_n01_asp1_c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7T2BGXX_n01_asp1_c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23779201
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT729110.30661669414376036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG25100.029.4253743
CGTATGC25750.028.95434644
CTCGTAT26050.027.94916342
TATGCCG30950.023.86299546
TCTCGTA39150.019.04420541
CGCGGGT40100.018.956271
ATGCCGT46850.016.28723347
TCACGAT47800.015.88913635
ACGATCT54500.015.862937
ACACGTC50800.014.67468613
CACGATC51850.014.24344136
TTCCGAT36000.013.5151512
GCCGTCT57650.013.11393249
CGGGTCT72600.012.535583
ACGTCTG60300.012.36280515
ATCACGA63550.012.28098234
ATCTCGT61300.012.21966240
TCCGATC39550.011.6824833
GTATGCC65950.011.67643145
ACGGAAC61800.011.49612134
Produced by FastQC (version 0.11.2)