FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_edhg_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_edhg_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15782335
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGA18400.011.7977951
CACGAGT83650.011.50607668
GGCCACG90250.010.62605765
GGGGCAT15600.010.5487331
GGGCTCC90400.010.45345752
CCGGGGT10400.010.4365111
CATGCGC92600.010.4318528
CGGGCAT14900.010.33935451
GGCCATC96450.010.26935910
ATGCGCT95650.010.02596329
AACGGCC98100.010.02562762
TCATGCG97450.09.98449727
GCCACGA100800.09.9305466
CCCCCTT15550.09.9071631
GGAGGGC98350.09.786449
GCGGGGG13600.09.7829921
GGATAAC111600.09.7257811
GTGCACA99400.09.6829341
CGGGGGT12350.09.6391551
AGGGCTC102100.09.59832751
Produced by FastQC (version 0.11.2)