FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_edhg_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_edhg_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32757546
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1535030.4686034784168509No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1387170.42346578708917937No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT805310.24583953877375309No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG792930.24206025689470145No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA768110.2344833767462312No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA581600.17754687729050278No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC549640.16779034668836304No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC544160.16611744970151304No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG525970.16056453068859308No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC464010.141649804902968No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT461250.14080725094608734No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC454780.13883213351818233No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC445130.1358862474008279No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG438690.13392028816810636No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA430080.13129188615044607No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC422350.12893212452483468No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG409120.12489336044891763No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT386660.11803692498821494No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC382540.11677919951634962No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT369990.11294802119792491No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT367950.1123252639254479No Hit
CTGGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGG367620.11222452377842955No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA366660.11193146153255801No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT348450.1063724370561824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT175700.034.6768341
CTGACTC217200.028.266792
ACTCTAG228800.027.1864665
CTAGCAG226400.027.1619938
GACTCTA238550.026.4860464
TGACTCT267600.023.257253
TAGCAGA271450.022.8346849
CTCTAGC285800.021.862346
CTATCGA309500.019.84599117
TCTATCG315600.019.34044816
GTGCGCA324000.018.36392868
TGCGCAT335200.017.84447169
GGTGCGC345650.017.2540967
AGCAGAT360450.017.12823310
GCAGATC357550.017.11040711
CCGGGGT23600.017.0626981
TATCGAT370600.016.6966718
CGGGGGG30500.016.6467721
GCGCTTC672550.016.56952968
GATCTAT370600.016.51746614
Produced by FastQC (version 0.11.2)