FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_edhg_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_edhg_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16585079
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT14000.012.5042761
GTCCGCT10800.012.3190281
CGGGGGG12350.011.33991051
CCCCCTT13750.011.2038321
GGGGCCT9100.011.15766051
GGGGCAT15350.010.948371
GGGGTAT15150.010.6306981
CACGAGT87100.010.48783268
GGGCGCT6402.055458E-1010.3941791
GCCGGGG18850.010.2156951
GCCGGGT23750.010.17191
GGCCACG91650.09.81461365
GCCCGAT19650.09.7997891
CATGCGC95900.09.5619728
GCCCTAT13350.09.4414311
GCCCGTT13050.09.3901841
GCCCCTT15000.09.3365261
GCCCCAT18550.09.24844551
GGGGCAC12150.09.21786921
GGGGACT43350.09.2073011
Produced by FastQC (version 0.11.2)