FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_edhg_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_edhg_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19117805
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC951930.49792850172914727No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG881240.46095249951550404No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT565310.29569817246279056No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG522060.2730752824395897No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA417330.21829388886433354No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC367040.1919885677252174No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA356000.18621384620253215No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC354950.185664619970755No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG319560.16715308059685724No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT306300.1602171378984146No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA294620.15410764991064613No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC289540.1514504410940482No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC282220.14762154964965904No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC276740.14475511179238412No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC273010.14280405098807109No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG268240.14030899467799782No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT264890.13855670146232793No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG261230.13664225574013333No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA253110.1323949062143902No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT242510.12685033663644962No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT241310.12622264951441864No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC231040.12085069389503658No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT220560.11536889302929912No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC206490.10800926152348556No Hit
CTGGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGG199760.10448898291409499No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT198590.10387698797011478No Hit
GGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCT194200.10158069924868468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT109950.037.831051
CTGACTC137100.030.4841182
ACTCTAG146500.028.8160635
CTAGCAG147100.028.504358
GACTCTA151200.028.4290564
TGACTCT162250.026.255313
TAGCAGA173500.024.5100929
CTCTAGC177350.024.1390516
CTATCGA201400.020.76675417
TCTATCG208250.020.1340916
GATCTAT223700.018.68088314
ATCTATC229250.018.44246315
GCAGATC228650.018.33778811
AGCAGAT230550.018.30815710
AGATCTA236850.017.77647413
TATCGAT237800.017.67611318
GTGCGCA222800.017.24856868
TGCGCAT229500.016.80610569
TAGCATG248250.016.79136827
GTCGCCT26450.016.679081
Produced by FastQC (version 0.11.2)