FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_edhg_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_edhg_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18406365
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC956100.5194398785420152No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG862800.4687508913356874No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT513710.27909367221610565No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG483700.2627895296002225No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA421320.22889907920439476No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC338230.183757086203604No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA336320.18271940168523226No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC330160.17937273329090236No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG294470.15998270163609163No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC279810.1520180654898455No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC275210.14951893000057317No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA262860.14280929450220076No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC262640.14268977063097465No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT255360.13873461707403933No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT248060.1347685977106289No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG246200.1337580777084449No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG242360.13167184286522624No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT240510.13066675576627976No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC234000.12712993575863565No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA225620.12257716284556999No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC206980.11245023121077952No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT204260.11097248153016633No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT201370.10940237249451482No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT201010.10920678797796306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT104700.035.683761
CTGACTC128950.029.1275392
CTAGCAG137300.027.4033188
ACTCTAG139950.027.0390365
GACTCTA147400.025.8621184
TGACTCT156400.024.1946353
TAGCAGA165200.022.902279
CTCTAGC168550.022.6586696
CTATCGA185750.020.27421217
TCTATCG189600.019.65947216
AGCAGAT216750.017.50359210
GCAGATC215550.017.40633411
TATCGAT218400.017.35518618
GATCTAT220750.017.04387314
CCGGGGT14100.016.8866181
CGCTTCA424850.016.64934369
GCGCTTC424350.016.60297868
TAGCATG229150.016.34272427
GTGCGCA205900.016.30154268
ATCTATC234200.016.12478415
Produced by FastQC (version 0.11.2)