FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_edhg_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_edhg_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30715453
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG29400.012.861911
CGGGGGG24350.012.5097691
CGGGGGA32550.011.7247751
CGCGGGG20250.011.4116371
CACGAGT169800.011.1101668
GGCCACG177850.010.60716165
CCGGGGT25500.010.2979351
GGGGAAC45400.010.0257431
CATGCGC185550.09.97840428
GCCGGGG36300.09.9348121
GCCACGA197450.09.80244466
AACGGCC192750.09.73282462
GGGCTCC191300.09.69661652
CGCCCAT40550.09.6706621
ATGCGCT196400.09.51614929
GGAGGGC197050.09.44932749
GGATAAC219000.09.4487081
GCCCGAT34250.09.4049451
GAACGGC195400.09.40376761
GGGGAAT99450.09.4001661
Produced by FastQC (version 0.11.2)