FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18284877
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT819900.44840334446876506No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG519440.2840817578373647No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA500140.27352658702598875No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC461900.2526131294183713No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA272300.14892088144754814No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG265280.1450816431524259No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG244570.13375534328177324No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC239570.1310208430715722No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG238630.13050675703205442No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA237510.12989422898496938No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG215550.11788430406176646No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC203640.11137072456106759No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG200700.1097628384374694No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT200180.10947845041560848No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC190810.10435399702169175No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG187730.10266954489220792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT136200.043.3175281
CTGACTC164150.036.0381052
CTAGCAG177150.033.7254268
ACTCTAG182500.033.5854575
GACTCTA187300.032.9114654
TGACTCT194800.030.8175723
CTCTAGC209750.029.3388946
TAGCAGA210400.028.6450049
CTATCGA237950.025.06378217
TCTATCG248400.024.051716
GCAGATC264950.022.68098811
AGCAGAT265350.022.64673410
GATCTAT264150.022.52486414
ATCTATC265900.022.40294315
AGATCTA279050.021.37195613
TATCGAT281200.021.2585718
TAGCATG295000.020.1453527
TTCTAGC298350.019.88390224
GCATGAC298650.019.84054229
ATTCTAG299950.019.81278823
Produced by FastQC (version 0.11.2)