FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17889230
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT889320.49712592436901976No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG663930.3711339168874233No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA614580.34354748639265076No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC592770.33135579340195187No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG342330.191360947340942No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG304910.1704433337823931No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG303520.16966632996501246No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA300260.16784400446525646No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG274760.15358961788741046No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG268430.15005117604279222No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC268430.15005117604279222No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT268390.15002881622070932No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA267670.1496263394232172No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG258620.14456742967696207No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC254270.14213579902544715No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG232150.12977081741360585No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC229720.12841245822206993No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC204710.1144319794647394No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT202830.1133810678268433No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC202680.11329721849403245No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG188130.10516383321137915No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC186830.10443713899368502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT148750.044.604841
CTGACTC175950.038.095252
ACTCTAG193100.035.5648845
CTAGCAG192500.034.8180058
GACTCTA201500.034.273174
TGACTCT206000.032.878533
CTCTAGC221750.031.0330566
TAGCAGA222350.030.1915749
CTATCGA250550.026.6251417
TCTATCG256200.025.9969916
GCAGATC271000.024.65478711
AGCAGAT275950.024.39009510
GATCTAT276100.024.13639614
ATCTATC277600.024.08162515
AGATCTA287100.023.2723913
TATCGAT290050.023.10764518
TTCTAGC309300.021.43179924
ATTCTAG311250.021.25254623
TAGCATG315150.021.1121227
GCATGAC319000.020.79131529
Produced by FastQC (version 0.11.2)