FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17739991
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG607430.3424071635662047No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA588110.3315165154255152No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC566810.319509744959848No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT558390.314763406587974No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG355090.20016357392740503No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC257500.14515227206146836No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA242880.1369110051972405No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG239180.1348253220647068No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT238790.13460547978857487No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG215080.12124019679604123No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG214360.12083433413241304No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG209980.11836533626200824No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC208700.11764380263778038No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA208260.11739577545445204No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG204270.11514661986017918No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC194320.10953782332809527No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT186400.10507333402818525No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG183430.10339915054071898No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC177810.10023116697184345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT102800.040.6348881
CTGACTC132150.031.5996132
ACTCTAG139700.030.8202925
CTAGCAG137850.030.4932928
GACTCTA145300.029.849024
TGACTCT160600.026.4600943
TAGCAGA165200.025.6144089
CTCTAGC168950.025.4222056
CTATCGA185500.022.30119917
TCTATCG190350.021.80652216
GCAGATC209150.020.03057311
GATCTAT208000.019.95633914
GTGCGCA221300.019.94312368
ATCTATC209850.019.93039915
AGCAGAT215400.019.69309410
TGCGCAT227150.019.46027269
GGTGCGC231500.019.06436767
TATCGAT223100.018.6364918
AGATCTA223950.018.62858413
AGGTGCG241550.018.37290666
Produced by FastQC (version 0.11.2)