FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23198145
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT953290.4109337190538295No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG707040.30478299019167265No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA701960.3025931599272269No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC630220.2716682734761766No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG394430.17002652582781944No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA337450.14546421707425314No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG333090.14358475645358712No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG331930.14308471647194204No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT315720.1360970887974017No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA305630.13174760309498884No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG298010.12846285769832028No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC286550.1235228075348266No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC284230.12252272757153643No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG282120.121613172087682No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG279380.12043204316552034No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG272580.11750077430760089No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC265270.11434966028533748No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT240380.10362035412745285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT164450.043.2236821
CTGACTC204600.034.9527172
ACTCTAG217400.033.5085
CTAGCAG214400.033.3185358
GACTCTA221800.033.0008164
TGACTCT241500.029.9896163
CTCTAGC256900.028.901046
TAGCAGA252700.028.2964949
CTATCGA282800.025.01175917
TCTATCG290950.024.3713916
ATCTATC318250.022.36894415
GATCTAT318700.022.32637814
GCAGATC320200.022.3089911
AGCAGAT324100.022.09467710
TATCGAT335150.021.21973418
AGATCTA338350.021.08110813
TAGCATG355800.019.860627
TTCTAGC357650.019.79679924
GCATGAC361200.019.57320229
ATTCTAG362250.019.53570723
Produced by FastQC (version 0.11.2)