FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25658584
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA740780.28870650071726484No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT664780.25908678358868126No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG549420.21412717085245234No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC500710.19514327057175096No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA409290.15951386873102585No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA343110.13372133084195137No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG328880.12817542854274422No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT321100.12514330486826553No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC278560.108564057938661No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT276780.10787033298485997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT136350.035.744671
CTGACTC187350.026.2127062
ACTCTAG197750.025.6316155
CTAGCAG194650.025.550948
GACTCTA206350.024.9873684
TGACTCT234800.021.6612643
TAGCAGA235500.021.3120639
CTCTAGC245200.020.814226
AGGGGCG295700.018.15649224
TAGGGGC298000.018.05162823
CTATCGA270950.018.04520417
GGGGCGG302900.017.72494325
TCTATCG278500.017.68174616
CGTAGGG307250.017.51928721
GTAGGGG308950.017.38917422
CGTTCAC307700.017.33510266
GGGTGCC310750.017.31080412
GTCTGGG317800.016.9493068
GCAGATC297350.016.83162511
TCGTAGG322950.016.78687520
Produced by FastQC (version 0.11.2)