FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28030072
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1092770.38985629433987895No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG917140.3271985887157193No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC854380.30480835011768787No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA834690.2977837516792679No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG468200.16703489024216564No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG405770.14476238234421945No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG386130.13775562189066087No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA369050.13166216626200605No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG355740.1269136946919009No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC350470.12503357108750918No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC344500.12290371569505779No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT342220.12209030358537787No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG329920.11770215930947306No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG328980.1173668051940787No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA327050.1166782589784286No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG317640.1133211502275128No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC312330.11142675623523193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT191750.041.2150651
CTGACTC242100.032.80612
CTAGCAG247000.032.477188
ACTCTAG253800.032.287815
GACTCTA259700.031.6752514
TGACTCT284250.028.4221153
CTCTAGC296150.027.7059066
TAGCAGA293400.027.3409989
CTATCGA328900.024.2091617
TCTATCG337650.023.46781316
GCAGATC368200.021.69141411
GATCTAT368000.021.61772214
ATCTATC371550.021.43020415
AGCAGAT377650.021.3894310
AGATCTA389400.020.49238813
TATCGAT394100.020.31918718
TAGCATG395900.020.0325327
GCATGAC405400.019.5196529
CTAGCAT408300.019.48408126
TTCTAGC406150.019.47509224
Produced by FastQC (version 0.11.2)