FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7627015
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT361260.4736584364918648No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG246040.322590161419638No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC213790.28030625349497806No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA213590.28004402770939874No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG131620.1725707894897283No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA129340.16958141553412442No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG115020.15080604928664754No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA114340.14991448161567794No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG112190.14709555442070063No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC111480.14616465288189415No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC103250.13537406180530653No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT99490.13044421703641595No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG95290.12493747553925093No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG93470.12255122089047943No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC88230.11568090530830215No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT87010.11408132801626848No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG79410.1041167481642556No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG77230.1012584871014414No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC77190.10120604194432553No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC76730.10060292263749317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT58600.043.7971231
CTGACTC72400.035.825372
ACTCTAG77600.034.5532955
GACTCTA81000.033.9672164
CTAGCAG77550.033.4431848
TGACTCT86500.030.269093
CTCTAGC89950.029.6535176
TAGCAGA88250.029.5465669
CTATCGA104900.024.65618517
TCTATCG107100.024.18238816
AGCAGAT114700.022.82432610
GCAGATC115350.022.57404711
GATCTAT114000.022.56506314
ATCTATC118050.021.93929715
AGATCTA118250.021.84270713
TATCGAT121850.021.48488418
TTCTAGC127750.020.24592624
GCATGAC129250.019.95667529
TAGCATG129850.019.91849727
ATTCTAG131500.019.64195423
Produced by FastQC (version 0.11.2)