FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262635
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG182200.3462143964002824No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT170520.3240201914060162No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC168050.3193267251101397No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA155910.2962584332753459No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG100730.19140601618770825No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG78550.14925982896400758No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC73980.14057596622224416No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA72460.13768767927093556No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG70540.1340393168061247No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT69380.13183509781696812No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC67350.12797771458594412No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG65780.12499441819544771No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG64170.1219351142536011No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA64110.12182110292657576No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC62710.11916083862931783No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT62300.118381761227978No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG57130.10855778521596121No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG53550.10175510937011592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT32350.039.6113931
CTGACTC40150.031.9928952
CTAGCAG41200.031.0917038
ACTCTAG43150.030.4189435
GACTCTA44150.029.8089494
TGACTCT48900.026.7699853
TAGCAGA49000.026.5712269
CTCTAGC51900.025.6277126
CTATCGA58250.022.17192617
TCTATCG58200.022.13104816
GATCTAT63400.020.42648914
GCAGATC64300.020.41197611
AGCAGAT65000.020.24561510
GTGCGCA60250.020.21542268
ATCTATC64700.019.85377315
TGCGCAT61700.019.79725669
AGATCTA66950.019.44720313
GGTGCGC64150.018.98660767
TATCGAT69500.018.73349418
TAGCATG71300.017.96637327
Produced by FastQC (version 0.11.2)