FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21832309
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1007510.46147661248290317No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA780090.3573098933328582No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG569250.26073742360462193No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA461180.21123739133593247No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC449220.20575927172888586No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA408440.18708053280118012No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG336660.15420265442377168No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG288550.13216650607134592No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC258050.11819638499986418No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT255690.11711541825466101No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC225760.10340637813435125No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC222630.1019727230866877No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG222570.1019452408813012No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG222060.10171164213551576No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT219560.10056655024441072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT164600.046.6519171
CTGACTC212100.036.4223672
ACTCTAG225350.034.980925
GACTCTA228250.034.6740264
CTAGCAG227800.033.740028
TGACTCT243550.032.135883
CTCTAGC262750.030.2016186
TAGCAGA263250.029.4758979
CTATCGA318300.024.02561817
TCTATCG323100.023.63630116
AGCAGAT344850.022.5010710
GCAGATC346050.022.28139311
GATCTAT350750.021.92290514
ATCTATC353650.021.73323615
AGATCTA366750.020.9471613
TATCGAT371800.020.52106518
TAGCATG401250.019.00692627
TTCTAGC407000.018.67781824
CAGATCT414600.018.63929712
CTAGCAT410100.018.61369526
Produced by FastQC (version 0.11.2)