FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11790247
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG439260.37256216939305853No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT423970.3595938236069185No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA407750.3458366902745973No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC332210.2817667857170422No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG239330.2029898101371413No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT179460.1522105516534132No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA177890.1508789425700751No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG173970.1475541606549888No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG173200.14690107849309686No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA173020.1467484099357715No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC165160.14008188293256282No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG159170.1350014126082346No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG150490.1276393955105436No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG146060.1238820526830354No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC130690.11084585420475077No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG125040.10605375782203715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT71050.043.4192241
CTGACTC91450.033.9517972
ACTCTAG96450.033.426815
CTAGCAG97150.032.2077568
GACTCTA100750.032.1037144
TGACTCT110750.028.6672733
CTCTAGC113050.028.4566886
TAGCAGA116300.026.9648289
CTATCGA134050.023.15865317
TCTATCG137350.022.50031516
GCAGATC149400.020.92023711
AGCAGAT153800.020.5721510
GATCTAT151600.020.50086214
ATCTATC152350.020.30813415
AGATCTA159450.019.57928513
TATCGAT159250.019.53777718
GTGCGCA156550.019.0032568
GCGCGCC62850.018.65550862
TGCGCAT160550.018.52979769
TCTAGCA246700.018.471737
Produced by FastQC (version 0.11.2)