FastQCFastQC Report
Thu 12 May 2016
HN7MLBGXX_n01_bzip1wt_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7MLBGXX_n01_bzip1wt_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20620052
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1114280.5403866100822636No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA674170.32694873902354854No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG497900.24146398854862247No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA430900.20897134498011938No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC418960.20318086491731444No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA407440.19759407008284943No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG363230.1761537749759312No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG325400.15780755548046146No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT274420.13308404847863622No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG272100.13195893007447312No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC252900.12264760535036479No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG251570.12200260212728853No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT239460.11612967804348892No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC231910.11246819358166507No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG227740.11044589024314778No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG223850.10855937705685709No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC218390.10591146908843878No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC218360.10589692014355734No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC218040.10574173139815553No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG215300.10441292776565259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT176050.046.342051
CTGACTC221100.037.1258742
ACTCTAG232150.036.2492455
GACTCTA241300.035.265944
CTAGCAG234950.035.2458658
TGACTCT253150.032.9512523
CTCTAGC266550.031.7943746
TAGCAGA267100.031.0426659
CTATCGA330450.024.85767217
TCTATCG334900.024.57962416
AGCAGAT346600.024.15416510
GCAGATC349550.023.64993711
GATCTAT354900.023.3227814
ATCTATC361800.022.93597215
AGATCTA368750.022.4941413
TATCGAT375650.021.95979318
CAGATCT412550.020.1738812
TTCTAGC411150.019.96190524
ATTCTAG418200.019.6253423
TAGCATG418600.019.60672827
Produced by FastQC (version 0.11.2)