FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n02_islbtn2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n02_islbtn2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39225499
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3547880.9044830761745057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG167750.026.0367820
GTACGGG172750.025.14005321
GAGTACG175100.025.06207819
AGAGTAC273500.024.6102518
TACGGGG114450.024.32295222
CGCAGAG288650.023.42753615
ACGCAGA319800.021.29662514
AGTGGTA321700.021.2417764
GTGGTAT337650.020.2894345
CAGAGTA338750.020.14480417
AACGCAG341250.019.92729613
GCAGTGG346200.019.8297982
AGCAGTG351650.019.8190251
GGTATCA350500.019.706347
GCAGAGT353150.019.33433716
TGGTATC365450.018.7554426
TCAACGC375950.018.16140711
GTATCAA398600.017.2937098
ATCAACG404700.016.9308710
CAACGCA415950.016.42320312
Produced by FastQC (version 0.11.2)