FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n02_islbtn1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n02_islbtn1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30496280
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2354750.7721433565011864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA488060.16003919166534408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA45500.030.858547
AGTACGG133900.025.43036520
GCCGTAT58050.025.31557348
GAGTACG135750.025.23628419
GTACGGG137050.024.96828821
TACGGGG86650.024.88111122
AGAGTAC229250.024.72544918
CGCAGAG236800.024.08446715
AGTGGTA257000.022.0661724
ACGCAGA263850.021.75916114
GTAGGGA74550.021.10246519
GCAGTGG270150.021.069682
GTGGTAT272900.020.8563695
GGTCGCC77650.020.83888244
CAGAGTA273050.020.83504717
AACGCAG274700.020.79883813
AGCAGTG285950.020.1728461
TGTAGGG79700.020.12846418
GCAGAGT287250.020.02274516
GGTATCA290050.019.8405697
Produced by FastQC (version 0.11.2)