FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n02_f8z2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n02_f8z2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23993465
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1982090.8260957723280069No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262720.10949648164614824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG109700.028.55206721
GAGTACG110800.028.45955719
AGAGTAC184400.027.6712618
TACGGGG76900.026.86945222
AGTACGG117950.026.85137220
CGCAGAG195850.026.1257715
AGTGGTA218850.023.7220334
GCAGAGT219650.023.43630816
TCAACGC219200.023.34178511
ACGCAGA220650.023.31445314
CAGAGTA223150.023.19081917
GTGGTAT238350.021.9265
GGTATCA236000.021.840937
AACGCAG237700.021.69942513
GCAGTGG242450.021.4714162
TGGTATC239900.021.4536176
AGCAGTG245250.021.3830591
ATCAACG246500.020.9106310
CAACGCA247500.020.81220212
GTATCAA257450.020.1150158
Produced by FastQC (version 0.11.2)