FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n02_f8z1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n02_f8z1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34061121
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2556930.750688739809826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG112900.023.4040621
AGAGTAC184250.023.19975518
GAGTACG116100.023.00002519
TACGGGG83700.021.92518222
CGCAGAG196700.021.59248415
AGTACGG122650.021.5748620
CAGAGTA238950.018.11991917
ACGCAGA235950.018.10295914
AGTGGTA237650.018.100354
GCAGAGT244350.017.6218616
TCAACGC242300.017.5995611
AACGCAG253200.017.01910813
GGTATCA259450.016.7018957
GTGGTAT267150.016.2823935
GAGTACT104050.016.14756620
TGGTATC270700.016.017896
TCCGATC115550.015.5534276
GCAGTGG283100.015.4271142
CAACGCA281000.015.26165312
ATCAACG287950.015.03705410
Produced by FastQC (version 0.11.2)