FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n02_f8pax372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n02_f8pax372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38174134
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2794140.7319458772791021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG172050.027.18728321
GAGTACG174150.027.08098219
AGAGTAC286400.026.65804918
CGCAGAG296800.025.64424915
AGTACGG183350.025.571620
TACGGGG118500.025.52951622
CAGAGTA337900.022.74819417
AGTGGTA342250.022.4761054
ACGCAGA342400.022.43054214
TCAACGC354250.021.7086711
GCAGAGT356500.021.52414516
AACGCAG357350.021.45282413
GTGGTAT367800.021.02735
GGTATCA374050.020.7805567
TGGTATC377100.020.5087286
GCAGTGG382450.020.4665432
ATCAACG393050.019.7236710
AGCAGTG404800.019.5428051
CAACGCA394150.019.50233812
GTATCAA406850.019.062848
Produced by FastQC (version 0.11.2)